• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Hairpin probes for real-time assay of restriction endonucleases

Thumbnail
Full Text
Hairpin probes for real-time assay of restriction endonucleases.htm (407bytes)
Date
2002
Author
Zhang, YY
张永有
Li, QG
李庆阁
Liang, JX
Zhu, YB
Collections
  • 生命科学-已发表论文 [5901]
Show full item record
Abstract
New fluorogenic probes for restriction endonucleases based on molecular beacons were developed. These hairpin probes were single-stranded oligonucleotides that had stem-and-loop structure and carried 5'-fluorophor moiety and 3'-quencher moiety. Their stem sequences were designed as recognition sites for restriction endonucleases and loop sequences were unrelated nucleotides. Upon cleavage by endonuclease, these probes became fluorescent and thus could trace the enzyme activity continuously. Two probes were designed for BglII and NcoI, respectively, and each was labeled with a fluorophor of different color. The results showed that the two probes could specifically assay for the corresponding enzymes either individually or simultaneously in a real-time mode. Considering the simplicity, quantification and high throughput, these probes could be extended to other applications such as drug screening, protein-nucleic acid interaction study and searching for small molecule DNA cleavage agents.
Citation
ACTA BIOCHIMICA ET BIOPHYSICA SINICA,vol34,issue3,pp329-332
URI
http://dx.doi.org/doi:cnki:ISSN:0582-9879.0.2002-03-011
https://dspace.xmu.edu.cn/handle/2288/11065

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies