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dc.contributor.authorLuo, W
dc.contributor.authorPeng, XX
dc.contributor.author彭宣宪
dc.contributor.authorWang, SY
dc.contributor.author王三英
dc.date.accessioned2011-10-28T08:53:24Z
dc.date.available2011-10-28T08:53:24Z
dc.date.issued2002
dc.identifier.citationFEMS Microbiology Letters 216 (2002) 39^42zh_CN
dc.identifier.issn0378-1097
dc.identifier.urihttp://dx.doi.org/doi:10.1111/j.1574-6968.2002.tb11411.x
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11047
dc.description.abstractInfections with bacteria producing shiga toxin are responsible for widespread disease and for the death of a large number of people. In the present study, we have developed a rapid method of high specificity for the detection of Shigella dysenteriae by combining immuno-capture of the bacteria and polymerase chain reaction (PCR) amplification of their toxin gene. We compared the sensitivity of our new method, referred to as immuno-capture toxin gene PCR (iTGPCR), with a conventional TGPCR (cTGPCR) method in detecting S. dysenteriae. Approximately 100 colony forming units (CFU) of bacteria in a volume of 400 mul were divided into 20 tubes with 5 CFU (20 mul). One group of 10 tubes was analyzed by iTGPCR and the other by cTGPCR amplification. All were positive in the 10 tubes using iTGPCR but, in contrast, only half were positive in the 10 tubes with the cTGPCR method. This method was used to detect S. dysenteriae type I in sewage samples without the normal tedious preparation methods. These findings suggest that iTGPCR gives enhanced test sensitivity, and allows determination of pathogen serotype, and differentiation of toxin-producing strains from non toxin-producing strains. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectShigella spp.zh_CN
dc.subjectbacterial diagnosiszh_CN
dc.subjectimmuno-capturezh_CN
dc.subjectshiga toxinzh_CN
dc.subjectShigella dysenteriaezh_CN
dc.titleIdentification of shiga toxin-producing bacteria by a new immuno-capture toxin gene PCRzh_CN
dc.typeArticlezh_CN


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