Enhancement ot antibody detection in cancer using panel of recombinant tumor-associated antigens
Zhang, JY（W.M. Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, California ）
Casiano, CA（Department of Biochemistry and Microbiology, Center for Molecular Biology and Gene Therapy, Loma Linda University School of Medicine, Loma Linda, California ）
Koziol, JA（Division of Biomathematics, Department of Molecular and Experimental Medicine, The Scripps Research Institute）
Chan, （W.M. Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, California ） EKL
Tan, EM（W.M. Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, California ）
- 生命科学－已发表论文 
Prawn White Spot Bacilliform Virus (WSBV) is a major pathogen that causes prawn diseases. In this study, we examined the sequence of WSBV genome DNA in the shrimp Penaeus japonicus, P. vannamei, P. Monodon, P. chinensis and Metapenaeus ensis through successive PCR amplification of the DNA fragments in the whole WSBV genome. We found a sequence deletion hotspot in the WSBV genome that is 305,107 bp in length. The sizes of the deleted fragments were 4.6, 4.8 or 8.1 kbp depending on the species of prawn. Since the mortality of shrimp infected by the intact WSBV was always significantly higher than that of shrimp infected by DNA fragment-deleted WSBV, we suggest that this deletion be somehow linked to the virulence of the virus itself. This result may lead to the discovery of the molecular mechanism of the pathogenicity of WSBV in shrimps. (C) 2002 Elsevier Science B.V. All rights reserved.