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dc.contributor.authorSu, WJ
dc.contributor.author苏文金
dc.contributor.authorSong, SY
dc.contributor.author宋思扬
dc.contributor.authorLong, MN
dc.contributor.author龙敏南
dc.contributor.authorLiu, GM
dc.date.accessioned2011-10-28T01:36:38Z
dc.date.available2011-10-28T01:36:38Z
dc.date.issued2003
dc.identifier.citationJournal of Biotechnology 105 (2003) 227–233zh_CN
dc.identifier.issn0168-1656
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.jbiotec.2003.07.001
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/11022
dc.description.abstractTo improve detection efficiency and result accuracy, four screening primer pairs, four identifying primer pairs, one common primer pair and corresponding probes were designed for the development of multiplex polymerase chain reaction/membrane hybridization assay (MPCR-MHA) for detection of the foreign genes insert in genetically modified organisms (GMOs). After detecting condition and parameter were optimized and determined, MPCR reactions were developed for amplifying several target genes simultaneously in one tube. Primers were labeled with biotin at the 5'-end; biotinylated MPCR products were detected by hybridization to the oligonucleotide probes immobilized on a membrane with subsequent colorimetric detection to confirm hybridization. The testing of screening primers can judge whether the sample contains GMOs, and that of identifying primers can further judge what kinds of trait genes are contained in the sample. We detected nine soybean samples, six maize samples, seven potato samples and two rice samples by the MPCR-MHA method; at the same time we also detected them with single PCR-MHA method. The results between two methods have good consistency. (C) 2003 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectgenetically modified organismszh_CN
dc.subjectmultiplex PCRzh_CN
dc.subjectmembrane hybridization assayzh_CN
dc.titleMultiplex polymerase chain reactionmembrane hybridization assay for detection of genetically modified organismszh_CN
dc.typeArticlezh_CN


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