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Fluorescence enhancement method for the determination of nucleic acids using cationic cyanine as a fluorescence probe

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Fluorescence enhancement method for the determination of nucleic acids using cationic cyanine as a fluorescence probe.pdf (119.3Kb)
Date
2004
Author
Zhu, CQ
Zhuo, SJ
Zheng, H
Chen, JL
Li, DH
李东辉
Li, SH
Xu, JG
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  • 生命科学-已发表论文 [5901]
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Abstract
A fluorescence enhancement method with a cationic cyanine as a probe was developed for the determination of nucleic acids. Under the experimental conditions, the fluorescence enhancement of cyanine (lambda(ex)/lambda(em) = 524/591.5 nm) was observed in the presence of DNA. The calibration graphs were linear over the range of 0.01 - 15 mug mL(-1) for both calf thymus DNA (CT DNA) and fish sperm DNA (FS DNA). The limits of detection were 0.005 and 0.007 mug mL(-1) for CT DNA and FS DNA, respectively. The method was applied to the determination of DNA in synthetic and real samples and satisfactory results were obtained. A possible fluorescence enhancement mechanism was also studied.
Citation
Analyst. 2004 Mar;129(3):254-8. Epub 2004 Jan 23
URI
http://dx.doi.org/doi:10.1039/b313463f
https://dspace.xmu.edu.cn/handle/2288/11011

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