Fluorescence enhancement method for the determination of nucleic acids using cationic cyanine as a fluorescence probe

Abstract

A fluorescence enhancement method with a cationic cyanine as a probe was developed for the determination of nucleic acids. Under the experimental conditions, the fluorescence enhancement of cyanine (lambda(ex)/lambda(em) = 524/591.5 nm) was observed in the presence of DNA. The calibration graphs were linear over the range of 0.01 - 15 mug mL(-1) for both calf thymus DNA (CT DNA) and fish sperm DNA (FS DNA). The limits of detection were 0.005 and 0.007 mug mL(-1) for CT DNA and FS DNA, respectively. The method was applied to the determination of DNA in synthetic and real samples and satisfactory results were obtained. A possible fluorescence enhancement mechanism was also studied.