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dc.contributor.authorJi, NN
dc.contributor.authorPeng, B
dc.contributor.authorWang, GZ
dc.contributor.authorWang, SY
dc.contributor.author王三英
dc.contributor.authorPeng, XX
dc.contributor.author彭宣宪
dc.date.accessioned2011-10-25T00:32:09Z
dc.date.available2011-10-25T00:32:09Z
dc.date.issued2004
dc.identifier.citationJ Microbiol Methods. 2004 Jun;57(3):409-13zh_CN
dc.identifier.issn0167-7012
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.mimet.2004.02.010
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10993
dc.description.abstractA universal primer PCR (UPPCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens. The results show that this method is efficient at amplifying the conserved regions of bacterial 16S rRNA genes with universal primers and can detect causative bacterial pathogens rapidly. Six species of bacteria from fisheries (Pseudomonas fluorescens, Vibrio anguillarum, Aeromonas hydrophila, Vibrio fluvialis, Providencia rettgeri and Aeromonas sobria) were examined. Our results indicate that the approach we undertook can be adopted not only for axenic bacterial populations but also for mixed communities as well. Furthermore, we were able to achieve the rapid detection of multiple bacteria a single in sample. In addition, UPPCR-DGGE was shown to be better than previously reported UPPCR-single-stranded conformation polymorphism (SSCP)-based methods for the rapid detection of bacterial pathogens. (C) 2004 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectfish pathogenszh_CN
dc.subjectuniversal primerzh_CN
dc.subjectPCR-DGGEzh_CN
dc.subjectPCR-SSCPzh_CN
dc.subject16S rDNAzh_CN
dc.titleUniversal primer PCR with DGGE for rapid detection of bacterial pathogenszh_CN
dc.typeArticlezh_CN


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