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dc.contributor.authorZhang, GL
dc.contributor.authorWang, GZ
dc.contributor.authorWang, SY
dc.contributor.author王三英
dc.contributor.authorLi, QF
dc.contributor.author李祈福
dc.contributor.authorOuyang, GL
dc.contributor.author欧阳高亮
dc.contributor.authorPeng, XX
dc.contributor.author彭宣宪
dc.date.accessioned2011-10-23T03:28:53Z
dc.date.available2011-10-23T03:28:53Z
dc.date.issued2004
dc.identifier.citationInt J Biochem Cell Biol. 2004 Aug;36(8):1613-23zh_CN
dc.identifier.issn1357-2725
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.biocel.2004.01.021
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10989
dc.description.abstractHuman gastric carcinoma BGC-823 cells underwent morphological differentiation and cell cycle arrest in vitro when treated with 5 mM hexamethylene bisacetamide (HMBA) for 48 h. To further understand the mechanism of HMBA-induced differentiation, proteomic methodologies were applied to screen and identify altered proteins involved in the commitment of BGC-823 cells to differentiate. Five distinct altered proteins were acquired by two-dimensional (2-D) PAGE and were consequently identified as ras-related protein rab-35 (Rab-35), splice truncated isoform of transmembrane protease, serine 3 (serine TADG-12), regulator of G-protein signaling 1 (RGS1), ret finger protein-like 1 (RFPL1) and F-actin capping protein alpha-3 subunit (GSG3) by analysis of mass spectrograph. Of the five proteins, serine TADG-12 down-regulated under the detectable level after HMBA treatment, Rab-35, RGS1 and RFPL1 sharply up-regulated within the HMBA-induced BGC-823 cells, and GSG3, appearing in both treated and untreated cells, remarkably increased within BGC-823 cells after HMBA stimulation. Our results implicate that the molecular mechanism of BGC-823 cell differentiation in response to HMBA may involved in complex processes including a signaling network linking vesicle transport, actin cytoskeleton remodeling except for morphology differentiation, cell cycle G1 arrest. (C) 2004 Elsevier Ltd. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDzh_CN
dc.subjectHMBAzh_CN
dc.subjectinduction differentiationzh_CN
dc.subjecthuman gastric carcinoma BGC-823 cellszh_CN
dc.subjectproteomicszh_CN
dc.titleApplying proteomic methodologies to analyze the effect of hexamethylene bisacetamide (HMBA) on proliferation and differentiation of human gastric carcinoma BGC-823 cellszh_CN
dc.typeArticlezh_CN


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