Changes of plasma membrane ATPase activity, membrane potential and transmembrane proton gradient in Kandelia candel and Avicennia marina seedlings with various salinities
- 生命科学－已发表论文 
The salt-secreting mangrove, Avicennia marina, and non-salt-secreting mangrove, Kandefia candel were cultivated in sand with various salinities (0parts per thousand, 10parts per thousand, 20parts per thousand, 30parts per thousand, 40parts per thousand) for 60 d. Plasma membrane vesicles of high-purity in leaves and roots of A. marina add K. candel seedlings were obtained by two-phase partitioning. The function of the plasma membranes, the activity of ATPase, membrane potential and transmembrane proton gradient, at various salinities were investigated. The results showed that within a certain range of salinity( A. marina and roots of K. candel: 0-30parts per thousand; leaves of K. candel: 0-20parts per thousand), the activity of ATPase increased with increasing salinity, while high salinity (above 30parts per thousand or 20parts per thousand) inhibited ATPase activity. In comparison with A. marina, K. candel appeared to be more sensitive to salinity. The dynamics of membrane potential and transmembrane proton gradient in leaves and roots of A. marina and K. candel seedlings were similar to that of ATPase. When treated directly by NaCl all the indexes were inhibited markedly: there was a little increase within 0-10parts per thousand( K. candel) or 0-20parts per thousand(A. marina) followed by sharp declining. It indicated that the structure and function of plasma membrane was damaged severely.