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dc.contributor.authorHuang, HQ
dc.contributor.author黄河清
dc.contributor.authorXiao, ZQ
dc.contributor.authorChen, X
dc.contributor.authorLin, QM
dc.contributor.authorCai, ZW(Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong)
dc.contributor.authorChen, P
dc.date.accessioned2011-10-20T01:12:17Z
dc.date.available2011-10-20T01:12:17Z
dc.date.issued2004
dc.identifier.citationBiophysical Chemistry,Volume 111, Issue 3, 1 November 2004, Pages 213-222zh_CN
dc.identifier.issn0301-4622
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.bpc.2004.06.002
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10956
dc.description.abstractThe ferritin consists of a protein shell constructed of 24 subunits and an iron core. The liver ferritin of Sphyrna zygaena (SZLF) purified by column chromatography is a protein composed of eight ferritins containing varying iron numbers ranging from 400 +/- 20 Fe3+/SZLF to 1890 +/- 20 F3+/SZLF within the protein shell. Nature SZLF (SZLF(N)) consisting of holoSZLF and SZLF with unsaturated iron (SZLF(UL)) to have been purified with polyacrylamide gel electrophoresis (PAGE) exhibited five ferritin bands with different pI values ranging from 4.0 to 7.0 in the gel slab of isoelectric focusing (IEF). HoloSZLF purified by PAGE (SZLFE) not only had 1890 +/- 20 Fe3+/SZLFE but also showed an identical size of iron core observed by transmission electron microscopy (TEM). Molecular weight of similar to 21 kDa for SZLFE subunit was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Four peaks of molecular ions at mass/charge (m/z) ratios of 10611.07, 21066.52, 41993.16, and 63555.64 that come from the SZLFE were determined by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS), which were identified as molecular ions of the ferritin subunit (M+) and its polymers, namely, [M](2+), [M](+), [2M](+), and [3M](+), respectively. Both SZLFE and a crude extract from shark liver of S. zygaena showed similar kinetic characteristics of complete iron release with biphasic behavior. In addition, a combined technique of visible spectrometry and column chromatography was used for studying ratio of phosphate to Fe3+ within the SZLFE core. Interestingly, this ratio maintained invariable even after the iron release, which differed from that of other mammal ferritins. (C) 2004 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectliver ferritinzh_CN
dc.subjectsharkzh_CN
dc.subjectMALDI-TOF MSzh_CN
dc.subjectkinetics of iron releasezh_CN
dc.subjectcompositionzh_CN
dc.subjecttumorzh_CN
dc.titleCharacteristics of structure, composition, mass spectra, and iron release from the ferritin of shark liver (Sphyrna zygaena)zh_CN
dc.typeArticlezh_CN


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