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dc.contributor.authorChen, Ying
dc.contributor.author陈莹
dc.contributor.authorYao, Minna
dc.contributor.author姚闽娜
dc.contributor.authorTang, Yaoji
dc.contributor.author唐尧基
dc.contributor.authorLi, Yaoqun
dc.contributor.author李耀群
dc.date.accessioned2011-10-13T14:36:37Z
dc.date.available2011-10-13T14:36:37Z
dc.date.issued2005
dc.identifier.citationSPECTROSCOPY AND SPECTRAL ANALYSIS,2005,25(12):2048-2051zh_CN
dc.identifier.issn1000-0593
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10918
dc.description.abstractA new method of quantitative determination for serum albumin in aqueous solution has been developed by measuring total internal reflection synchronous fluorescence at the solid/liquid interface. The combination of bovine serum album in (BSA) and mesotetrakis (4-sulphonatophenyl) porphyrin (TPPS) adsorbed onto the glass surface produced a synchronous fluorescence signal at 421 nm. At pH 4.25, the signal intensity of BSA adsorbed on the interface was proportional to the BSA concentration in bulk solution. The linear range of 1.0-8.0 mu g center dot mL(-1) and the detection limit of 0.94 mu g center dot mL(-1) were obtained. The human serum samples were determined with satisfactory results.zh_CN
dc.language.isozhzh_CN
dc.publisherBEIJING UNIV PRESSzh_CN
dc.subjecttotal internal reflectionzh_CN
dc.subjectsynchronous fluorescencezh_CN
dc.subjectTPPSzh_CN
dc.subjectproteinzh_CN
dc.titleDetermination of protein with TPPS by total internal reflection synchronous fluorescence spectroscopyzh_CN
dc.typeArticlezh_CN


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