• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
  •   DSpace Home
  • 化学化工学院
  • 化学化工-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Determination of protein with TPPS by total internal reflection synchronous fluorescence spectroscopy

Thumbnail
Full Text
卟啉全内反射同步荧光法测定蛋白质.pdf (249.7Kb)
Date
2005
Author
Chen, Ying
陈莹
Yao, Minna
姚闽娜
Tang, Yaoji
唐尧基
Li, Yaoqun
李耀群
Collections
  • 化学化工-已发表论文 [14469]
Show full item record
Abstract
A new method of quantitative determination for serum albumin in aqueous solution has been developed by measuring total internal reflection synchronous fluorescence at the solid/liquid interface. The combination of bovine serum album in (BSA) and mesotetrakis (4-sulphonatophenyl) porphyrin (TPPS) adsorbed onto the glass surface produced a synchronous fluorescence signal at 421 nm. At pH 4.25, the signal intensity of BSA adsorbed on the interface was proportional to the BSA concentration in bulk solution. The linear range of 1.0-8.0 mu g center dot mL(-1) and the detection limit of 0.94 mu g center dot mL(-1) were obtained. The human serum samples were determined with satisfactory results.
Citation
SPECTROSCOPY AND SPECTRAL ANALYSIS,2005,25(12):2048-2051
URI
https://dspace.xmu.edu.cn/handle/2288/10918

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies