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dc.contributor.authorPeng, XX
dc.contributor.author彭宣宪
dc.contributor.authorWu, YJ
dc.contributor.authorChen, JG
dc.contributor.authorWang, SY
dc.contributor.author王三英
dc.date.accessioned2011-10-11T11:45:57Z
dc.date.available2011-10-11T11:45:57Z
dc.date.issued2004
dc.identifier.citationPROTEOMICS,vol4,issue12,pp3989-3997zh_CN
dc.identifier.issn1615-9853
dc.identifier.urihttp://dx.doi.org/doi:10.1002/pmic.200300864
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10896
dc.description.abstractProteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from the proteomic study. Fifteen APR-related proteins with sixteen spots (PLA with two spots) on a 2-DE map were identified in this study. Furthermore, six were known acute phase proteins including galactose-binding lectin (GBL), lysozyme, C3, CD59, double PLA and 50s ribosomal protein; while ATP kinase, zinc finger protein 183, alpha-neurotoxin homology, angiostatin, serine/threonine kinase, metalloproteinase inhibitor, regulator of G-protein 4, cryptdin-9 and disintegrin trigranin were found by our lab to be APR-related proteins. In addition, our results suggest that proteomes with low molecular weight can be characterized by 2-DE with a Tris-tricine system followed by mass spectrometry.zh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-V C H VERLAG GMBHzh_CN
dc.subjectacute phase proteinzh_CN
dc.subjectacute phase reactionzh_CN
dc.subjectloachzh_CN
dc.subjectskinzh_CN
dc.titleProteomic approach to identify acute phase response-related proteins with low molecular weight in loach skin following injuryzh_CN
dc.typeArticlezh_CN


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