Preparation and characterization of chitosan membrane for solid phase microextraction technique

Abstract

Chitosan is a kind of natural polymers containing plenty of amido and hydoxy. The chitosan membrane is tough, and since the partition coefficient of chlorophyll between chitosan membrane and water is as high as 9 090, the chitosan membrane may be easily manufactured as solid-phase microextraction membrane for the analysis of chlorophyll. The system may reach a complete equilibrium in 80 min. Then the membrane can be desorbed completely in 30 min in 5% NaOH with ultrasonic. Both IR and XRD indicated that hydrogen bonds between the molecules of chitosan were weakened and amido and hydoxy we re considered as the keys during the process of extracting chlorophyll.