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dc.contributor.authorZhu, CQ
dc.contributor.authorZhuo, SJ(College of Chemistry and Materials Science, Anhui Normal University, Wuhu)
dc.contributor.authorZheng, H
dc.contributor.authorChen, JL(College of Chemistry and Materials Science, Anhui Normal University, Wuhu)
dc.contributor.authorLi, DH
dc.contributor.author李东辉
dc.contributor.authorLi, SH
dc.contributor.authorXu, JG
dc.date.accessioned2011-10-08T01:43:30Z
dc.date.available2011-10-08T01:43:30Z
dc.date.issued2005
dc.identifier.citationPERGAMON-ELSEVIER SCIENCE LTD,Volume 61, Issue 4, February 2005, Pages 743-748zh_CN
dc.identifier.issn1386-1425
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.saa.2004.05.029
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10870
dc.description.abstractA new method based on near-infrared (near-IR) fluorescence recovery was presented for the determination of nucleic acids. This method employed a two-regent system composed of anionic tetracarboxy aluminum phthalocyanine, (AlC4Pc) and polycationic poly-lysine. The fluorescence of AlC4Pc, with the maximum excitation and emission wavelengths at 620 and 701 nm, respectively, was quenched by polylysine with a proper concentration, but recovered by adding nucleic acids. Under optimal conditions, the recovered fluorescence was in proportional to the concentration of nucleic acids. The linear ranges of the calibration curves were 5-200 ng mL(-1) for both calf thymus DNA (ctDNA) and fish sperm DNA (fsDNA) with the detection limit of 2.6 ng mL(-1) for ctDNA and 2.1 ng mL(-1) for fsDNA. The relative standard deviation (n = 6) was 1.9 and 1.3% for 50 ng mL(-1) ctDNA and fsDNA, respectively. The proposed method was applied to the determination of nucleic acids in synthetic samples with satisfactory results. (C) 2004 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDzh_CN
dc.subjectnear-IR fluorescencezh_CN
dc.subjecttetracarboxy aluminum phthalocyaninezh_CN
dc.subjectpoly-lysinezh_CN
dc.subjectnucleic acidszh_CN
dc.titleDetermination of nucleic acids based on shifting the association equilibrium between tetracarboxy aluminum phthalocyanine and poly-lysinezh_CN
dc.typeArticlezh_CN


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