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dc.contributor.author吕意华
dc.contributor.author周进
dc.contributor.author郑伟
dc.contributor.author高岩
dc.contributor.author俞志明
dc.contributor.author郑天凌
dc.date.accessioned2016-05-17T03:01:34Z
dc.date.available2016-05-17T03:01:34Z
dc.date.issued2015-3-28
dc.identifier.citation厦门大学学报(自然科学版),2015,(2):40-45
dc.identifier.issn0438-0479
dc.identifier.otherXDZK201502006
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/108463
dc.description.abstract雪卡毒素作为一种脂溶性藻毒素近年来日益引起人们的重视,冈比亚藻(gAMbIErdISCuS SPP.)是雪卡毒素的主要产生藻.不同的冈比亚藻具有不同的生长特性和产毒性能,且存在地域差异性,因而对冈比亚藻的监测存在较大挑战.建立一种快速简单地鉴定冈比亚藻及其种群结构的方法,是基础科研和社会公众关心的共性问题.为此,以加勒比海的10种冈比亚藻为受试生物,应用限制性片段长度多态性(rESTrICTIOn frAgMEnT lEngTH POlyMOrPHISM,rflP)分析技术,通过生物软件分析发现受试藻的限制性内切酶ACI I具有良好的特异性和灵敏性,可有效区分目前已鉴定的10种冈比亚藻.验证实验中,通过与分离自野外环境的多株冈比亚藻进行酶切电泳和dnA测序,证实了ACI I的有效性和可靠性.结果表明,该ACI I分子具有检测自然环境中冈比亚藻属性和追踪其种群结构变化的潜力,对雪卡毒素的预判与风险评估具有重要的借鉴意义.
dc.description.abstractCiguatera fish poisoning(CFP)is a serious seafood poisoning syndrome caused by the consumption of seafood contaminated with ciguatoxins.The toxic organisms most commonly associated with CFP are benthic dinoflagellates in the genus Gambierdiscus.To establish molecular monitoring for the Gambierdiscus community in tropical seas,we analyzed the restriction fragment length polymorphism(RFLP)of large subunit ribosomal(LSU)rDNA sequences of D1-D2 region from ten Gambierdiscus species and several environmental samples.Results showed that the restriction enzyme AciI was able to identify LSU rDNA heterogeneities of Gambierdiscus species,repeatedly and sensitively.Therefore,the assay in this study could be established to monitor Gambierdiscusspecies in the environments and trace the changes of Gambierdiscus community in the ocean.
dc.description.sponsorship国家海洋公益性行业专项(201305016;201305022); 国家基金委-山东省联合基金项目(U1406403)
dc.language.isozh_CN
dc.subject雪卡毒素
dc.subject冈比亚藻
dc.subject限制性片段长度多态性
dc.subjectAci I
dc.subjectD1-D2
dc.subjectciguatera
dc.subjectGambierdiscus spp.
dc.subjectrestriction fragment length polymorphism(RFLP)
dc.subjectAci I
dc.subjectD1-D2
dc.title一种基于RFLP分析的冈比亚藻检测方法的建立
dc.title.alternativeLSU rDNA Based on RFLP Assays for Distinguishing Species of Gambierdiscus spp.
dc.typeArticle


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