Fluorescent characterization off peroxynitrite-induced thiyl radicals
- 化学化工－已发表论文 
A spin-labeling fluorophore (Ac-Tempo(.)), formed by combining with an acridine with a paramagnetic nitroxide (4-hydroxyl-2, 2, 6, 6-tetramethylpiperidinyl-1-oxy), was explored for peptide-based thiyl radicals generated through the reaction between gluththione and peroxynitrite. Ac-Tempo(.), a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after interaction with thiyl radicals, and could be employed to probe peptide-based thiyl radicals.