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dc.contributor.authorSong, KK
dc.contributor.authorChen, QX
dc.contributor.author陈清西
dc.contributor.authorWang, Q
dc.contributor.author王勤
dc.contributor.authorQiu, L
dc.contributor.authorHuang, H
dc.date.accessioned2011-10-02T07:29:46Z
dc.date.available2011-10-02T07:29:46Z
dc.date.issued2005
dc.identifier.citationJ Enzyme Inhib Med Chem. 2005 Jun;20(3):239-43.zh_CN
dc.identifier.issn1475-6366
dc.identifier.urihttp://dx.doi.org/doi:10.1016/0921-5093(94)09633-8
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10805
dc.description.abstractTyrosinase (EC 1.14.18.1) catalyzes both the hydroxylation of ryrosine into o-diphenols and the oxidation of o-diphenols into o-quinones which form brown or black pigments. Here, the inhibitory effects of 4-vinylbenzaldehyde and 4-vinylbenzoic acid on the activity of mushroom tyrosinase have been investigated. The results showed that both 4-vinylbenzaldehyde and 4-vinylbenzoic acid could inhibit both monophenolase activity and diphenolase activity of the enzyme. For the monophenolase activity, 4-vinylbenzoic acid could lengthen the lag time, but 4-vinylbenzaldehyde could not. Both 4-vinylbenzaldehyde and 4-vinylbenzoic acid decreased the steady-state activity, and the IC50 values were estimated as 93 mu M and 3.0 mM for monophenolase activity, respectively. For the diphenolase activity, the inhibitory capacity of 4-vinylbenzaldehyde was stronger than that of 4-vinylbenzoic acid, and the IC50 values were estimated as 23 mu M and 0.33 mM, respectively. Kinetic analyses showed that inhibition by both compounds was reversible and their mechanisms were mixed-II type; their inhibition constants were also determined and compared.zh_CN
dc.language.isoenzh_CN
dc.publisherTAYLOR & FRANCIS LTDzh_CN
dc.subjecttyrosinasezh_CN
dc.subjectmonophenolase activityzh_CN
dc.subjectdiphenolase activityzh_CN
dc.subject4-vinylbenzaldehydezh_CN
dc.subject4-vinylbenzoic acidzh_CN
dc.subjectinhibition kineticszh_CN
dc.titleInhibitory effects of 4-vinylbenzaldehyde and 4-vinylbenzoic acid on the activity of mushroom tyrosinasezh_CN
dc.typeArticlezh_CN


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