冬季不同海区拟穴青蟹可溶蛋白和糖含量比较

Abstract

采用生物化学方法测定了福建厦门海区和浙江宁海海区冬季拟穴青蟹鳃、肝胰腺和肌肉中可溶性蛋白和可溶性糖的含量。结果显示,厦门海区拟穴青蟹冬季可溶性蛋白含量在鳃(4.02Mg/Ml)、肝胰腺(3.12Mg/Ml)和肌肉(4.35Mg/Ml)中显著高于宁海海区拟穴青蟹鳃(2.49Mg/Ml)、肝胰腺(2.22Mg/Ml)和肌肉(3.07Mg/Ml)相应器官组织中可溶性蛋白含量,差异极显著(P<0.01)。而冬季可溶性糖的含量,厦门海区拟穴青蟹鳃(1.10Mg/Ml)、肝胰腺(1.26Mg/Ml)和肌肉(0.68Mg/Ml)与宁海海区拟穴青蟹鳃(1.12Mg/Ml)、肝胰腺(0.97Mg/Ml)和肌肉(0.73Mg/Ml)相应器官组织之间比较,均无显著性差异(P>0.05)。说明不同生化成分在不同海区拟穴青蟹冬季适应中有不同的生理调控。可溶性蛋白的变化在一定程度上反应了生理代谢酶的动态变化。

In this study,biochemical methods were adopted to determine the contents of soluble protein and saccharides in gills,hepatopancreas and muscles of mud crab Scylla paramamosain collected from Xiamen waters and Ninghai waters in winter.There were significantly higher levels of soluble proteins in the gills(2.49 mg/ml),hepatopancreas(3.12 mg/ml) and muscle(4.35 mg/ml) in the mud crab in Xiamen waters than those(2.49 mg/ml,2.22 mg/ml,and 3.07 mg/ml) in Ninghai waters(P<0.01).In the soluble saccharides,there was no significant difference in different organs or tissues of the crab in the different waters(P>0.05).It was demonstrated that the different biochemical components in the crab performed the different physiological regulation strategies to adapt the low temperature in winter.The changes in the soluble protein levels were partly reflected the dynamic changes in the physiological metabolism.