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dc.contributor.authorKong, B
dc.contributor.authorHuang, HQ
dc.contributor.author黄河清
dc.contributor.authorLin, QM
dc.contributor.authorCai, ZW
dc.contributor.authorChen, P
dc.date.accessioned2011-10-01T15:38:34Z
dc.date.available2011-10-01T15:38:34Z
dc.date.issued2005
dc.identifier.citationAppl Biochem Biotechnol. 2005 Aug;126(2):133-48zh_CN
dc.identifier.issn0273-2289
dc.identifier.urihttp://dx.doi.org/doi:10.1385/ABAB:126:2:133
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10798
dc.description.abstractWe describe a protein reactor consisting of native liver ferritin of Dasyatis akajei (DALE) and a dialysis bag. Our goal was to study a ferritin reactor for its capacity to trap various heavy metal ions (M2+) in flowing seawater. The reactor is sensitive and inexpensive and can be operated by nonprofessional technicians. A positive relationship between the number of trapped M2+ with the DALF reactor and its concentration in the flowing seawater was observed. Both the pH in the medium and the phosphate content within the ferritin cavity strongly affected trapping capacity. It was found that the ferritin released its phosphate compound directly with a shift in pH without the need for releasing reagent, which differs from the phosphate release characteristics of horse spleen ferritin, as previously described. This behavior evidently makes the trapping capacity with the ferritin reactor weaken, indicating that this trapping capacity is tightly connected to its phosphate compound. Our study shows that a self-regulation ability of the ferritin shell rather than its phosphate compound plays an important role in controlling the rate and capacity of trapping M2+. The ferritin reactor was constructed to monitor the contamination level of M2+ in flowing seawater. Our preliminary data along with fieldwork indicate that the DALF reactor is an analytical means for effectively monitoring the contamination level of M2+ in flowing seawater.zh_CN
dc.language.isoenzh_CN
dc.publisherHUMANA PRESS INCzh_CN
dc.subjectDasyatis akajeizh_CN
dc.subjectheavy metal ionszh_CN
dc.subjectferritin reactorzh_CN
dc.subjectphosphate effectzh_CN
dc.subjectseawaterzh_CN
dc.subjectcontamination levelzh_CN
dc.titleEffect of pH and phosphate on trapping capacity of various heavy metal ions with ferritin reactor in flowing seawaterzh_CN
dc.typeArticlezh_CN


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