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dc.contributor.authorHuang, XH
dc.contributor.authorChen, QX
dc.contributor.author陈清西
dc.contributor.authorWang, Q
dc.contributor.author王勤
dc.contributor.authorSong, KK
dc.contributor.authorWang, J
dc.contributor.authorSha, L
dc.contributor.authorGuan, X
dc.date.accessioned2011-10-01T14:52:42Z
dc.date.available2011-10-01T14:52:42Z
dc.date.issued2005
dc.identifier.citationELSEVIER SCI LTD,Volume 94, Issue 1, January 2006, Pages 1-6zh_CN
dc.identifier.issn0308-8146
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.foodchem.2004.09.008
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10796
dc.description.abstractThe inhibition kinetics of alkylbenzoic acids on the diphenolase activity of mushroom tyrosinase have been investigated. The results show that the alkylbenzoic acids assayed can lead to reversible inhibition of the enzyme; furthermore, o-toluic acid and m-toluic acid are mixed-type inhibitors and p-alkylbenzoic acids are uncompetitive inhibitors. The inhibition constants have been determined. For these p-alkylbenzoic acids, the inhibition strength follows the order: p-toluic acid < p-ethylbenzoic acid < p-propylbenzoic acid < p-isopropylbenzoic acid < p-tert-butylbenzoic acid < p-butylbenzoic acid < p-pentylbenzoic acid < p-hexylbenzoic acid < p-heptylbenzoic acid < p-octylbenzoic acid, indicating that the hydrophobic p-alkyl group played an important role in the inhibition of the enzyme. The inhibitory effects were potentiated with increasing lengths of the hydrocarbon chains. The inhibitory effects of o-toluic acid and p-isopropylbenzoic acid on the monophenolase activity have also been studied. The results show that both o-toluic acid and p-isopropylbenzoic acid can lengthen the lag time and decrease the steady-state activity of the enzyme. (c) 2004 Elsevier Ltd. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCI LTDzh_CN
dc.subjectmushroom tyrosinasezh_CN
dc.subjectdiphenolase activityzh_CN
dc.subjectmonophenolase activityzh_CN
dc.subjectalkylbenzoic acidszh_CN
dc.subjectinhibitionzh_CN
dc.subjectkineticszh_CN
dc.titleInhibition of the activity of mushroom tyrosinase by alkylbenzoic acidszh_CN
dc.typeArticlezh_CN


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