Show simple item record

dc.contributor.authorLin, JC
dc.contributor.authorXie, XL
dc.contributor.authorGong, M
dc.contributor.authorWang, Q
dc.contributor.author王勤
dc.contributor.authorChen, QX
dc.contributor.author陈清西
dc.date.accessioned2011-10-01T09:36:39Z
dc.date.available2011-10-01T09:36:39Z
dc.date.issued2005
dc.identifier.citationInternational Journal of Biological Macromolecules,Volume 36, Issue 5, 28 September 2005, Pages 327-330zh_CN
dc.identifier.issn0141-8130
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.ijbiomac.2005.06.012
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10793
dc.description.abstractbeta-N-acetyl-D-glucosaminidase (NAGase, EC.3.2.1.52), a composition of the chitinases, catalyzes the cleavage of N-acetylglucosamine polymers into N-acetylglucosamine. In this paper, the effects of mercuric ion on the activity of NAGase from Penaeus vannamei for the hydrolysis of pNP-NAG have been studied. The results show that HgCl2 can lead to irreversible inactivation to this enzyme. The inactivation process follows a first-order reaction and the inactivation rate constants have been determined. The relationship between the inactivation rate constants and HgCl2 concentration has been studied and the result shows that only one molecule of HgCl2 binds to the enzyme molecule to lead the enzyme lose its activity. Moreover, the conformational changes of the enzyme inactivated by HgCl2 were studied by following changes in the intrinsic fluorescence emission and ultraviolet absorption spectra. (c) 2005 Elsevier B.V. All rights reserved.zh_CN
dc.language.isoenzh_CN
dc.publisherELSEVIER SCIENCE BVzh_CN
dc.subjectbeta-N-acetyl-D-glucosaminidasezh_CN
dc.subjectPenaeus vannameizh_CN
dc.subjectmercuric chloridezh_CN
dc.subjectinactivation kineticszh_CN
dc.subjectconformational changeszh_CN
dc.titleEffects of mercuric ion on the conformation and activity of Penaeus vannamei beta-N-acetyl-D-glucosaminidasezh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record