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dc.contributor.authorYang, YH
dc.contributor.authorQiu, YL
dc.contributor.authorXie, CT
dc.contributor.authorTian, HQ
dc.contributor.author田惠桥
dc.contributor.authorZhang, Z
dc.contributor.authorRussell, SD
dc.date.accessioned2011-10-01T09:04:56Z
dc.date.available2011-10-01T09:04:56Z
dc.date.issued2005
dc.identifier.citationSexual Plant Reproduction ,Volume 18, Number 2, 47-53zh_CN
dc.identifier.issn0934-0882
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s00497-005-0248-x
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10788
dc.description.abstractPrior research has indicated that the two sperm cells of Nicotiana tabacum are dimorphic, suggesting that they may participate in preferential fertilization during in vivo fusion with the egg and central cells. To probe the mechanism of potential preferential fertilization in this plant, it will be necessary to use modern sensitive molecular techniques. For this purpose, two individual populations of two sperm cells, constituting the Svn (associated with the vegetative nucleus) and Sua (unassociated with the vegetative nucleus), were isolated in the thousands from tobacco pollen tubes with a micromanipulator as a preliminary step toward research on gametic recognition using molecular techniques. Microelectrophoresis of paired sperm cells from a single pollen tube was conducted at different developmental stages. Sperm cells isolated from 1-, 2-, 3- and 4-cm stylar lengths migrated to the negative pole, with the Sua displaying significantly greater electrophoretic mobility than the Svn, reflecting a more positively charged cell surface on the Sua. The sperm cells isolated from 1-cm style are very sensitive to electron potential in an electrophoretic field, presumably reflecting that they are still in a young state. Differences in cell surface charge between the Sua and Svn may be related with cell fate during fertilization.zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectmicroelectrophoresiszh_CN
dc.subjectNicotiana tabacumzh_CN
dc.subjectpreferential fertilizationzh_CN
dc.subjectsperm cellszh_CN
dc.subjectsurface chargezh_CN
dc.titleIsolation of two populations of sperm cells and microelectrophoresis of pairs of sperm cells from pollen tubes of tobacco (Nicotiana tabacum)zh_CN
dc.typeArticlezh_CN


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