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dc.contributor.author郑洪
dc.contributor.author李东辉
dc.contributor.author吴敏
dc.contributor.author许金钩
dc.contributor.author陈秋影
dc.date.accessioned2016-05-17T02:56:21Z
dc.date.available2016-05-17T02:56:21Z
dc.date.issued1999
dc.identifier.citation高等学校化学学报,1999,(4):62-64
dc.identifier.issn0251-0790
dc.identifier.otherGDXH904.014
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/107695
dc.description.abstract染料结合法测定蛋白质在生化及临床分析中已有广泛应用,常用的染料有溴甲酚绿[1]、溴酚蓝[2]、考马斯亮蓝[3]及铬天青S[4]等,这些染料的最大吸收波长均处于可见光区.近年来,近红外染料越来越引起人们的注意[5],因为它们的吸收及荧光光谱均处于近红外...
dc.description.abstractA new watersoluble nearIR cyanine dye was synthesized and its structure was characterized by 1H NMR, MS and IR spectra.The dye was used as a probe to establish a novel nearIR spectrophotometric method for the determination of trace amount of serum protein.The dye has a maximum absorption wavelength at 776 nm, its absorbance decreases when it reacts with protein(for example, BSA).The reaction of the dye with BSA was carried out in 002 mol/L HAcNaAc buffer system(pH 40).When 3010-6 mol/L cyanine was used, the linearity of this method was over the range of 100102190103 ng/mL by determination of HSA and globulin, and the detection limit was 50 ng/mL.
dc.description.sponsorship国家自然科学基金;教育部材料与生命过程分析科学开放实验室资助
dc.language.isozh_CN
dc.subject近红外染料
dc.subject花菁
dc.subject血清蛋白质
dc.subject分光光度法
dc.subjectNearIR dye
dc.subjectCyanine
dc.subjectSerum protein
dc.subjectSpectrophotometry
dc.title合成的近红外花菁染料测定痕量血清蛋白质
dc.title.alternativeStudies on the Application of Synthesized NearIR Cyanine to the Determination of Trace Amounts of Protein
dc.typeArticle


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