仿生荧光免疫分析卵清白蛋白
Biomimetic Fluoroimmunoassay for Egg Albumin
Abstract
利用分子印记技术， 制备了卵清白蛋白（EA） 的人工抗体. 研究了其对抗原的特异性吸附能力， 在此基础上， 模拟竞争型免疫反应， 建立了卵清白蛋白的仿生荧光免疫分析方法. A gel mimetic antibody for egg albumin has been prepared by molecular imprinting and applied to develop a biomimetic fluoroimmunoassay for egg albumin.The format of the assay is analogous to that of the competitive fluoroimmunoassay.The specific binding between the mimetic antibody and antigen(egg albumin) was tested by using egg albumin-FITC,bovine serium albumin(BSA)-FITC and human serium albumin(HSA)-FITC conjugates as fluorescence probes,the results are satisfactory.