蔬菜叶片中叶绿素a和叶绿素b的一阶导数分光光度法测定
Determination of Chlorophyll a and Chlorophyllb in Vegetable Leaves by First Derivative Spectropho-tometry
Date
1994Collections
- 化学化工－已发表论文 [14469]
Abstract
本文提出用一阶异数分光光度法同时测定蔬菜叶片中叶绿素A和叶绿素b的含量。讨论了同时存在于蔬菜叶片中的类胡萝卜素对两者测定的影响，并提出用适当浓度的类胡萝卜素丙酮溶液作为参比溶液，用以消除类胡萝卜素对叶绿素测定的影响。 A First derivative spectrophotometric method ispresented For the determination of chlorophyll a andchlorophyll b in green vegetables.Chlorophyll a is de-termined at 671nm using an acetone solution as thereFerence solution.Chlorophyll b is determined at467nm using a carotenoid solutiOn as the reFerence so-lution, The linear correlation coeFFicients of standardcurves are equal to 0.9999747 and 0.9999859 Forchlorophyll a and chlorophyll b respectively.The re-coveries are between 100.5%and 101.8%For chloro-phyll a.