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dc.contributor.authorYang, SC
dc.contributor.author杨盛昌
dc.date.accessioned2011-09-16T07:16:40Z
dc.date.available2011-09-16T07:16:40Z
dc.date.issued2006
dc.identifier.citationJOURNAL OF RARE EARTHS ,2006, 24(2),pp243-247zh_CN
dc.identifier.issn1002-0721
dc.identifier.urihttp://dx.doi.org/doi:10.1016/S1002-0721(06)60102-6
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10745
dc.description.abstractA rapid and concentration-dependent generation of superoxide anion ( center dot O-2(-)), measured with a superoxide-specific Cypridina luciferin-derived chemiluminescent reagent, was observed when two lanthanide salts ( LaCl3 and GdCl3) were added to tobacco (Nicotiana tabacum) cell suspension culture. Addition of superoxide dismutase (480 U center dot ml(-1)) and Tiron (5 mu mol center dot L-1) to cell culture suspension decreases the level of lanthanide cation-induced center dot O-2(-) generation, suggesting that center dot O-2(-) generation is extra-cellular. Pretreatment of the cell culture Suspension with diphenylenelodonium (10 and 50 mu mol center dot L-1), quinacrine ( 1 and 5 mmol center dot L-1) and imidazol ( 10 mmol center dot L-1), inhibitors of NADPH oxidase, notably inhibits the generation of superoxide induced by lanthanide cation, implying the possible involvement of activation of NADPH oxidase. In addition, addition of SHAM ( 1 and 5 mmol center dot L-1), azide (0.2 and 1 mmol center dot L-1), inhibitor of peroxidase, has no influence on center dot O-2(-) generation.zh_CN
dc.language.isoenzh_CN
dc.publisherMETALLURGICAL INDUSTRY PRESSzh_CN
dc.subjectlanthanidezh_CN
dc.subjectoxidative burstzh_CN
dc.subjectBY-2 cellszh_CN
dc.subjectNADPH oxidasezh_CN
dc.subjectsuperoxide anionzh_CN
dc.subjectrare earthszh_CN
dc.titlePossible involvement of NADPH oxidase in lanthanide cation-induced superoxide anion generation in BY-2 tobacco cell suspension culturezh_CN
dc.typeArticlezh_CN


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