Possible involvement of NADPH oxidase in lanthanide cation-induced superoxide anion generation in BY-2 tobacco cell suspension culture
- 生命科学－已发表论文 
A rapid and concentration-dependent generation of superoxide anion ( center dot O-2(-)), measured with a superoxide-specific Cypridina luciferin-derived chemiluminescent reagent, was observed when two lanthanide salts ( LaCl3 and GdCl3) were added to tobacco (Nicotiana tabacum) cell suspension culture. Addition of superoxide dismutase (480 U center dot ml(-1)) and Tiron (5 mu mol center dot L-1) to cell culture suspension decreases the level of lanthanide cation-induced center dot O-2(-) generation, suggesting that center dot O-2(-) generation is extra-cellular. Pretreatment of the cell culture Suspension with diphenylenelodonium (10 and 50 mu mol center dot L-1), quinacrine ( 1 and 5 mmol center dot L-1) and imidazol ( 10 mmol center dot L-1), inhibitors of NADPH oxidase, notably inhibits the generation of superoxide induced by lanthanide cation, implying the possible involvement of activation of NADPH oxidase. In addition, addition of SHAM ( 1 and 5 mmol center dot L-1), azide (0.2 and 1 mmol center dot L-1), inhibitor of peroxidase, has no influence on center dot O-2(-) generation.