三维荧光光谱总体积积分法同时测定维生素B_2和B_6

Abstract

运用三维荧光光谱总体积积分法建立了一种测定维生素b2和b6的荧光分析新方法，该方法的灵敏度（信背比）较常规峰值法分别提高130和310倍，同时成功地应用于两者的同时测定.

In this paper, a novel Fluorescence method For the determination of vitamin B2 and vitamin B6 has been established using three-dimensional Fluorescence spectrometry by detect-ing the total Fluorescence intensity.The sensitivity of the method, the ratio of signal to blank noise, is nearly 130 and 310 times better than that of the conventional peak intensity method For the determination of vitamin B2 and vitamin B6, respectively.This method has been successFully used to determine vitamin B2 and vitamin B6 simultaneously.