Bioelectrocatalytic Oxidation of p Hydroxyphenol at Horseradish Peroxidase/Poly o phenylenediamine Membrane Electrode: A Kinetic Study
- 化学化工－已发表论文 
用酶的电化学固定化方法制备辣根过氧化物酶/聚邻苯二胺膜（HrP/PPd）电极.以对苯二酚和邻苯二胺在该酶电极上的氧化为模型体系，讨论了有机物生物催化氧化的一般动力学模式以及影响酶电极检测的因素.邻苯二胺可在辣根过氧化物酶的存在下发生聚合，因而它在HrP/PPd电极上的氧化过程比对苯二酚的复杂.测定了不同电位下对苯二酚在HrP/PPd电极上的反应动力学参数，并根据实验结果确定该有机物检测的合适工作电位.A horseradish peroxidase (HRP)/poly o phenylenediamine(PPD) membrane electrode was prepared by electrochemical immobilization of enzyme. The oxidations of p hydroxyphenol and o phenylenediamine as model systems were studied at the enzyme electrode, thereby the general kinetic scheme of bioelectrocatalytic oxidation of organic compounds and the Factors inFluencing on the detection with the HRP/PPD electrode were discussed. o Phenylenediamine was Found to be polymerized in the presence of HRP, hence its oxidation process at the HRP/PPD electrode is more complicated than that of p hydroxyphenol.The kinetic parameters of p hydroxyphenol oxidation at the enzyme electrode were measured at diFFerent potentials, and the optimum operational potential For p hydroxyphenol detection was determined.