离子色谱-积分脉冲安培检测法测定D-核糖

Abstract

采用离子色谱-积分脉冲安培检测法(IC-IPAd)测定d-核糖,采用CArbO PACTM PA10色谱柱进行分离,60 MMOl/l nA OH溶液为淋洗液,流速0.6 M l/MIn,柱温30℃,Ed3000安培检测器,Au工作电极,TI对电极,Ag/Ag Cl复合参比电极。以外标法定量,d-核糖质量浓度为3.60~43.2Mg/l范围内峰面积呈良好的线性关系(r2=0.9999),检出限及定量限分别为0.200、0.620 ng,精密度实验的相对标准偏差(rSd)为0.524%,平均回收率为101%。因此,IC-IPAd可用于d-核糖含量的测定,且操作简便、无需衍生、分离效果好、灵敏度高。

An analytical method was proposed and established for determination of D-ribose by ion chromatography with integrated pulsed amperometric detection(IC-IPAD).The separation was performed on a 250 mm×4 mm Carbo PacTM PA10 column at 30 ℃ by HPLC equipped with an ED3000 detector.The mobile phase was 60 mmol/L sodium hydroxide with a flow rate of 0.6 m L/min.The quantification was performed by an external standard approach.The concentration linear range for D-ribose was 3.60~43.2 mg/L(R2=0.9999).The relative standard deviation of precision was 0.524%.The detection and quantification limits for D-ribose were 0.200 ng and 0.620 ng,respectively.The average recovery ration for D-ribose was 101%.IC-IPAD was proved to be suitable for the determination of D-ribose with its convenience,rapidness,and sensitivity.