Show simple item record

dc.contributor.authorCheng, Yangjian
dc.contributor.authorNiu, Jianjun(Xiamen Center for Disease Control and Prevention)
dc.contributor.authorZhang, Yongyou
dc.contributor.authorHuang, Jianwei(Xiamen Center for Disease Control and Prevention)
dc.contributor.authorLi, Qingge
dc.contributor.author李庆阁
dc.date.accessioned2011-09-01T02:36:50Z
dc.date.available2011-09-01T02:36:50Z
dc.date.issued2006
dc.identifier.citationJournal of Clinical Microbiology, October 2006, p. 3557-3561, Vol. 44, No. 10zh_CN
dc.identifier.issn0095-1137
dc.identifier.urihttp://dx.doi.org/doi:10.1128/JCM.00713-06
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10716
dc.description.abstractArmored RNA has been increasingly used as both an external and internal positive control in nucleic acid-based assays for RNA virus. In order to facilitate armored RNA purification, a His(6) tag was introduced into the loop region of the MS2 coat protein, which allows the exposure of multiple His tags on the surface during armored RNA assembly. The His-tagged armored RNA particles were purified to homogeneity and verified to be free of DNA contamination in a single run of affinity chromatography. A fragment of severe acute respiratory syndrome coronavirus (SARS-CoV) genome targeted for SARS-CoV detection was chosen for an external positive control preparation. A plant-specific gene sequence was chosen for a universal noncompetitive internal positive control preparation. Both controls were purified by Co2+ affinity chromatography and were included in a real-time reverse transcription-PCR assay for SARS-CoV. The noncompetitive internal positive control can be added to clinical samples before RNA extraction and enables the identification of potential inhibitive effects without interfering with target amplification. The external control could be used for the quantification of viral loads in clinical samples.zh_CN
dc.language.isoenzh_CN
dc.publisherAMER SOC MICROBIOLOGYzh_CN
dc.titlePreparation of His-tagged armored RNA phage particles as a control for real-time reverse transcription-PCR detection of severe acute respiratory syndrome coronaviruszh_CN
dc.typeArticlezh_CN


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record