基于氮掺杂三维石墨烯/血红蛋白的丙烯酰胺电化学传感器制备及应用

Abstract

本研究利用氮掺杂三维石墨烯/血红蛋白(3d n-rgO/Hb)修饰的玻碳电极,实现对食品中丙烯酰胺(AA)的电化学快速检测。以3d n-rgO作为玻碳电极上电子传递材料,利用其疏松多孔、吸附能力强及电子传输通道多等优势,增强了AA与Hb的相互作用,从而降低Hb的电化学信号,实现对AA的响应。实验采用示差脉冲伏安法(dPV)检测AA,所测得氧化还原峰电位为-350MV,峰电流值随AA浓度增大而逐渐减小。在AA浓度为1.4x10-12~1.4x10-4 MOl/l范围内,峰电流强度与AA浓度呈现良好的线性关系(r2=0.9799),检出限(S/n=3)为1.0x10-12 MOl/l。研究表明该方法制备的涂层厚度均一且不易脱落,能够实现对实际样品中AA的检测。

Based on our previous research,three-dimensional N-doped graphene/hemoglobin(3D N-RGO/Hb) was prepared by hydrothermally assembled method.Benefiting from its excellent electrical conductivity,high surface area,biocompatibility and mechanical properties,3D N-RGO was used as support material of Hb,and a 3D N-RGO/Hb modified glassy carbon electrode(GCE)was then developed for the detection of acrylamide(AA).Differential pulse voltammogram(DPV)results showed that the 3D N-RGO/Hb modified GCE presented highly sensitive and stable sensing characteristics for AA detection.The analytical performance of the Nafion/Hb/N-RGO/GCE toward AA was evaluated in the linear response range from 1.4×10-12 to 1.4×10-4 mol/L,with a detection limit(S/N=3)of 1.0×10-12 mol/L.These results indicated that the proposed method was promising for the development of novel electrochemical sensing for AA detection.