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dc.contributor.authorWang, Sanying
dc.contributor.author王三英
dc.contributor.authorZhu, Rui
dc.contributor.authorPeng, Bo
dc.contributor.authorLiu, Mingming
dc.contributor.authorLou, Yun
dc.contributor.authorYe, Xintai
dc.contributor.authorXu, Zhengzheng
dc.contributor.authorLiu, Dong
dc.contributor.authorPeng, Xuanxian
dc.contributor.author彭宣宪
dc.date.accessioned2011-09-01T02:31:25Z
dc.date.available2011-09-01T02:31:25Z
dc.date.issued2006
dc.identifier.citationProteomics (2006) Volume: 6, Issue: 19, Pages: 5212-20zh_CN
dc.identifier.issn16159853
dc.identifier.urihttp://dx.doi.org/doi:10.1002/pmic.200500065
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10715
dc.description.abstractThe available Escherichia coli genome sequences offer an opportunity to further expand our understanding of this bacterium. In the current study, we present a rapid method for the isolation of bacterial alkaline proteins using acid incubation, purification and protein array by 2-DE, followed by protein identification using MS. Fifty-seven proteins were randomly chosen, in which 55 were identified by a database searching of MS data. The searching results showed that most of these alkaline proteins were involved in special functions within the cell, suggesting that alkaline proteome is an ideal fraction for an understanding of their special functions. Furthermore, alkaline proteomes were compared between the period of majority live bacteria (18-h culture), the period of similar amount of live and dead bacteria (30-h culture) and the period of majority dead bacteria (48-h culture). Six proteins were identified as differentially expressed targets, in which putative transcriptional regulator and superoxide dismutase genes were cloned and expressed for antiserum preparations. The antisera. were applied for the confirmation of results obtained from 2-DE. The presented data dearly reveal that alkaline proteome analysis by 2-DE with MS plays an important role in the understanding of protein functions within the cell, and six alkaline proteins are determined as key ones in an overgrowth-mediated growth cycle of E. coli.zh_CN
dc.language.isoenzh_CN
dc.publisherWILEY-V C H VERLAG GMBHzh_CN
dc.subject2D-PAGEzh_CN
dc.subjectalkaline proteinszh_CN
dc.subjectEscherichia colizh_CN
dc.subjectmetabolismzh_CN
dc.titleIdentification of alkaline proteins that are differentially expressed in an overgrowth-mediated growth arrest and cell death of Escherichia coli by proteomic methodologieszh_CN
dc.typeArticlezh_CN


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