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dc.contributor.authorChen, Su Hong
dc.contributor.authorLiao, Jing Ping
dc.contributor.authorKuang, An Xiu
dc.contributor.authorTian, Hui Qiao
dc.contributor.author田惠桥
dc.date.accessioned2011-09-01T02:11:19Z
dc.date.available2011-09-01T02:11:19Z
dc.date.issued2006
dc.identifier.citationPlant Cell Reports ,Volume 25, Number 11, 1138-1142zh_CN
dc.identifier.issn0721-7714
dc.identifier.urihttp://dx.doi.org/doi:10.1007/s00299-006-0189-3
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10712
dc.description.abstractThe two sperm cells of Torenia fournieri are dimorphic. The dimorphic character suggests that they might be preferentially involved in fertilization during in vivo fusion with the egg cell and central cell. To probe the mechanism of preferential fertilization, it is necessary to use the most current molecular techniques. For this purpose, populations of > 1000 individuals of the two dimorphic sperm cells, Sua (unassociated with the vegetative nucleus) and Svn (associated with the vegetative nucleus) were isolated from pollen tubes that had grown out of the cut ends of the styles. The two sperm cells released from pollen tubes remained attached to one another. When the two attached sperm cells were transferred into a solution containing 0.01% cellulose, 0.01% pectinase, and 5% mannitol, the connection between the two cells disappeared, and they were easily separated using a micromanipulator. The collection of these two individual populations containing over a thousand cells will permit research on gametic recognition at the molecular level.zh_CN
dc.language.isoenzh_CN
dc.publisherSPRINGERzh_CN
dc.subjectheterospermyzh_CN
dc.subjectdimorphic sperm cellszh_CN
dc.subjectTorenia fournierizh_CN
dc.titleIsolation of two populations of sperm cells from the pollen tube of Torenia fournierizh_CN
dc.typeArticlezh_CN


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