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Inhibitory kinetics of mercuric ion on the activity of beta-N-acetyl-D-glucosaminidase from green crab (Scylla serrata)

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Inhibitory kinetics of mercuric ion on the activity of beta-N-acetyl-D-glucosaminidase from green crab (Scylla serrata).htm (413bytes)
Date
2006
Author
Xie, Jin-Jin
Chen, Qing-Xi
陈清西
Zhang, Ji-Ping
Wang, Qin
王勤
Yang, Xue-Min
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  • 生命科学-已发表论文 [5901]
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Abstract
Chemical modification of p-chloromercuribenzoate (PCMB) on beta-N-acetyl-D-glucosaminidase (NAGase, EC 3.2.1.52) from green crab (Scylla serrata) has been studied. The results show that sulfhydryl group is essential for the activity of the enzyme. Inhibitory kinetics of the enzyme by mercuric chloride (HgCl2) has been studied using the kinetic method of the substrate reaction during inhibitor of enzyme. The kinetic results show that the inhibition of the enzyme by mercuric ion (Hg2+) at lower than 1.0 mu M is a reversible reaction with residual activity and the inhibition belongs to be competitive. The inhibition kinetics model of Hg2+ on the enzyme was set up and the microscopic rate constants were determined and the data obtained were well fitted with the model. It was also turned out that only one molecule of HgCl2 binds to the enzyme molecule to lead the enzyme lose its activity. The above results suggest that the cysteine residue is essential for activity and is situated at the active site of the enzyme. (c) 2005 Elsevier B.V. All rights reserved.
Citation
Int J Biol Macromol. 2006 Nov 15;39(4-5):159-64. Epub 2006 Jul 3.
URI
http://dx.doi.org/doi:10.1016/j.ijbiomac.2005.10.006
https://dspace.xmu.edu.cn/handle/2288/10699

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