基于石蜡庚烷溶液的低成本纸基微流控分析装置的制备及其应用

Abstract

介绍了一种新型的使用石蜡庚烷溶液制备纸基微流控分析装置的工艺,操作更加简单迅速,成本更加低廉。使用混合样品溶液进行测试,证明该装置可以完成对蛋白质、葡萄糖和PH值的同时检测,并可以用于定量分析。纸基微流控分析装置对样品检测的精度范围分别为:牛血清蛋白2.0x10-5~1.00x10-4MOl/l,葡萄糖0.02~0.1 MOl/l。并对加热处理过程中微流控通道壁的扩散现象进行了初步研究,发现加热前后通道壁厚度的变化呈线性规律。

Paper is an ideal substrate material for low-cost diagnostic devices and shows a great potential in the fabrication of microfluidic paper-based analytical devices( μPADs).As one of the hot research fileds,a variety of materials and techniques have been proposed to fabricate μPADs.In this paper,we introduced a cheap,simpler and faster fabrication procedure,in which paraffin-heptane solution was used,toward this target.It is confirmed that as-developed protocol could be used for the simultaneous diagnostics of protein, glucose and pH.In addition,it is also applicable for quantitative analysis.The detection ranges of fabricated μPAD for bovine serum albumin and glucose were 2.0 × 10- 5~ 1.00 × 10- 4mol / L and 0.02 ~ 0.1 mol / L, respectively.This paper also preliminarily studied the spreading phenomena of microfluidic channel barrier during heating treatment.We found that the thickness of barrier followed a linear discipline.