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dc.contributor.authorXie, Jin-Jin
dc.contributor.authorChen, Qing-Xi
dc.contributor.author陈清西
dc.contributor.authorWang, Qin
dc.contributor.author王勤
dc.contributor.authorSong, Kang-Kang
dc.contributor.authorQiu, Ling
dc.date.accessioned2011-08-27T08:22:45Z
dc.date.available2011-08-27T08:22:45Z
dc.date.issued2007
dc.identifier.citationPesticide Biochemistry and Physiology,Volume 87, Issue 1, January 2007, Pages 9-13zh_CN
dc.identifier.issn0048-3575
dc.identifier.urihttp://dx.doi.org/doi:10.1016/j.pestbp.2006.03.004
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10696
dc.description.abstractProphenol oxidase (PPO) (EC 1.14.18.1) is isolated from pupae of blowfly (Sarcophaga bullata) and purified by employing ammonium sulfate fractionation, ion-exchange chromatography on DEAE-cellulose, and get filtration through Sephadex G-100 column chromatography. The enzyme exists in a latent or inactive state. Cetylpyridinium chloride (CPC), a cationic detergent, is found to activate the PPO activity. The activation of the enzyme by CPC has first been studied by using the kinetic method of the substrate reaction described by Tsou. The results show that the enzyme is activated by a complexing scheme that has not been previously identified. The enzyme first reversibly and quickly binds CPC and then undergoes a slow reversible active course. The activation reaction is a single molecule reaction and the apparent activation rate constant is dependent on the CPC concentration with the function relationship fit with a hyperbola. The micro rate constants of activation and the association constant are determined from the measurements. Substrate binding does not affect the micro rate constants of activation by CPC. (c) 2006 Published by Elsevier Inc.zh_CN
dc.language.isoenzh_CN
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEzh_CN
dc.subjectprophenol oxidasezh_CN
dc.subjectblowfly pupaezh_CN
dc.subjectkineticzh_CN
dc.subjectactivationzh_CN
dc.subjectcetylpyridinium chloridezh_CN
dc.titleActivation kinetics of cetylpyridinium chloride on the prophenol oxidase from pupae of blowfly (Sarcophaga bullata)zh_CN
dc.typeArticlezh_CN


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