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dc.contributor.authorWang Zhong-An
dc.contributor.author王忠安
dc.contributor.authorWu-Juan
dc.date.accessioned2011-08-27T08:17:57Z
dc.date.available2011-08-27T08:17:57Z
dc.date.issued2006
dc.identifier.citationBANGLADESH JOURNAL OF BOTANY,35(1):189-192zh_CN
dc.identifier.issn0253-5416
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/10695
dc.description.abstractSperm cells of photoperiod-sensitive genic male-sterile rice (PGMR) were isolated from pollen tubes using two step osmotic shock with BSA and sucrose solution, which were separated and purified using a micromanipulator. The highest ratio of viable sperm cells was obtained when pollen grains cultured in a medium containing 1.5 mM BSA and 10% sucrose solution. Fluorescein diacetate (FDA) stain was used to determine the viable sperm cells. The size of two sperm cells of PGMR nongken 58S were different but the dimorphism of sperm cells in 58S needs to be confirmed by further work.zh_CN
dc.language.isoenzh_CN
dc.publisherBANGLADESH BOTANICAL SOCzh_CN
dc.subjectdiversityzh_CN
dc.subjectsperm cellzh_CN
dc.subjectphotoperiod-sensetivezh_CN
dc.subjectmale sterilezh_CN
dc.subjectOryza sativazh_CN
dc.titleDiversity of sperm cells of different size in photoperiod-sensitive genic male-sterile ricezh_CN
dc.typeArticlezh_CN


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