Diversity of sperm cells of different size in photoperiod-sensitive genic male-sterile rice

Abstract

Sperm cells of photoperiod-sensitive genic male-sterile rice (PGMR) were isolated from pollen tubes using two step osmotic shock with BSA and sucrose solution, which were separated and purified using a micromanipulator. The highest ratio of viable sperm cells was obtained when pollen grains cultured in a medium containing 1.5 mM BSA and 10% sucrose solution. Fluorescein diacetate (FDA) stain was used to determine the viable sperm cells. The size of two sperm cells of PGMR nongken 58S were different but the dimorphism of sperm cells in 58S needs to be confirmed by further work.