模拟移动床色谱制备纯化柚皮苷

Abstract

通过四区模拟移动床色谱制备纯化柚皮苷。采用高效液相色谱测试单柱的相关参数,通过三角形方法,确定线性条件下的完全分离操作范围。在该范围中选取操作条件进行柚皮苷的制备纯化。实验结果表明,当选取的一至四区流速分别为2Ml/MIn,1.35Ml/MIn,1.69Ml/MIn,0.69Ml/MIn,切换时间为35.5MIn时,通过面积归一法计算出,该系统制备的柚皮苷纯度可从粗品的82%提升到98%左右,主要杂质峰基本消失,满足柚皮苷的高纯度制备要求。柚皮苷的回收率为97%,产率为0.33Mg/MIn,溶剂耗量为4.85Ml/Mg。

The naringing was purified by four-zone simulated moving bed.The relative parameters were determined by HPLC and the complete separation region was found under linear condition with triangle theory.In this region,the oparation conditions were set.As the result,according to the area normalization method,the naringin purity increased to appoximate 98% from the 82% in crude sample by this system when flow rates of the four zone were 2mL/min,1.35 mL/min,1.69 mL/min,0.69 mL/min respectively and switching time was 35.5min.The main impurity was disappeared and this process could reach the separation requirment.The naringin recovery was 97%,productivity was 0.33mg/mL and desorbent consumption was 4.85mL/mg.