亮白曲霉代谢物中两种联苯化合物的分离鉴定

Abstract

对深海来源真菌亮白曲霉(ASPErgIlluS CAndIduS)进行固体发酵,并采用浸泡萃取法和中高压制备柱对代谢产物进行提取分离,从中分离得到化合物1和2,通过波谱解析确定化合物1为三联苯曲霉素(TErPHEnyllIn),化合物2为2,4-二甲氧基-1,10-二羟基对联三苯.对6种不同菌株进行抑菌实验,表明两化合物均具有抗菌活性.本实验是首次报道化合物2(2,4-二甲氧基-1,10-二羟基对联三苯),也是第一次从海洋真菌中分离得到化合物2.

The vast ocean area is a great treasure trove of resources,but the research and utilization is not enough,especially for deep-sea microorganisms.Deep-sea has specific environments,and the chance of obtaining novel structure is big.Deep-sea microorganisms have been focused on its significant economic value.Solid fermentation was performed using Aspergillus candicus from deepsea.The solid fermentation was extracted using soaking method and the metabolites were separated using medium-pressure preparative liquid chromatography column.Two compounds of antifungal bioactivity were obtained.Based on their spectroscopic data,their structures were elucidated.Compound 1 is terphenyllin.It′s a antioxidant and has cytotoxicity.Compound 2 is 2,4-dimethyl-1,10-dihydroxy-p-terphenyl,apotential antioxidant.It is the first report of compound 2,especially fromAspergillus candicus of deep-sea.