非衍生化/液相色谱-串联质谱法测定食品中的二硫代氨基甲酸酯类农药残留

Abstract

通过优化质谱、液相色谱和萃取缓冲液等条件,建立了一种简单、快速、灵敏测定食品中二硫代氨基甲酸酯的方法。在优化条件下,代森锌(EbdC)、丙森锌(PbdC)的线性范围为2~100μg/l,相关系数均不小于0.997。在花椰菜、萝卜和豌豆中EbdC和PbdC的检出限(S/n>3)为2μg/kg;萝卜和豌豆中Eb-dC和PbdC的定量下限(S/n>10)为10μg/kg,而花椰菜中EbdC和PbdC的定量下限(S/n>10)为15μg/kg。在花椰菜、萝卜和豌豆基质中加标5~100μg/kg的EbdC和PbdC时,测定加标回收率为83%~96%,相对标准偏差为4.4%~10.5%,方法可满足定量分析的要求。

A simple,fast and sensitive method was developed for the determination of dithiocarbamates,including tiezene(EBDC) and pestanal(PBDC),by optimizing the parameters of LC-MS/MS and extraction buffer.Under the optimized conditions,the calibration curves of EBDC and PBDC were linear in the range of 2-100 μg/L with correlation coefficients not less than 0.997.The limits of detection(LODs,S/N>3) of EBDC and PBDC were 2 μg/kg for cauliflower,turnip and pea,and the limits of quantitation(LOQs,S/N>10) of EBDC and PBDC were 10 μg/kg for turnip and pea,and 15 μg/kg for cauliflower.The recoveries in cauliflower,turnip and pea samples at spiked concentration levels of 5-100 μg/kg ranged from 83% to 96% with relative standard deviations(RSDs) of 4.4%-10.5%.The method could meet the requirement for the analysis of dithiocarbamates in foodstuffs.