丁酸梭菌产1,3-丙二醇的培养基优化

Abstract

为提高丁酸梭菌(ClOSTrIdIuM buTyrICuM)VPI 1718产1,3-丙二醇的能力,采用均匀设计试验和人工神经网络结合遗传算法对培养基进行优化,得到最优配方(g/l):废甘油70,酵母粉2.0,kH2PO4 0.6,k2HPO4 1.2,(nH4)2SO42.6,MgSO4 0.2;使用该培养基在5 l发酵罐中分批培养14 H丁酸梭菌后,1,3-丙二醇质量浓度为33.0g/l,转化率为55.3%,生产强度为2.36 g/(l·H),较优化前分别提高了32.5%,7.17%和33.3%.

In the present work, uniform design and artificial neural network linked genetic algorithm were applied to optimize 1,3-propanediol fermentation medium to enhance 1,3-propanediol production by Clostridium butyricum VPI 1718.The compositions of the optimized fermentation medium were as follows (g/L):raw glycerol 70, yeast extract 2.0, K2H PO4 1.2,KH2PO4 0.6,(NH4)2SO4 2.6,Mg SO4 0.2.When the C.butyricum was cultivated in the optimized medium for 14 h on a 5 L fermentor,the 1,3-propanediol concentration, yield and productivity were 33.0 g/L,55.3% and 2.36 g/(L·h),which were 32.5 % , 7.17 % and 35.6 % highter than that in the initial medium, respectively.