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dc.contributor.author庄灵习
dc.contributor.author段然
dc.contributor.author陈龙军
dc.contributor.author凌雪萍
dc.contributor.author卢英华
dc.date.accessioned2016-05-17T02:52:32Z
dc.date.available2016-05-17T02:52:32Z
dc.date.issued2011
dc.identifier.citation食品工业科技,2011,(11):141-144
dc.identifier.issn1002-0306
dc.identifier.otherSPKJ201111044
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/106602
dc.description.abstractα-1,6-葡聚糖酶是专一作用于α-1,6糖苷键产生小分子葡聚糖的一类水解酶,广泛的运用于制糖工业和啤酒工业中。采用PCr法扩增朱黄青霉(PEnICIllIuM MInIOluTEuM)C12114的α-1,6-葡聚糖酶基因,将其插入毕赤酵母表达载体PPIC9k。经SACI酶线性化电击转入毕赤酵母基因组,构建重组酵母gS115/PPIC9k-dEX。对构建成功的转化子进行1.5%的甲醇诱导表达,在30℃条件下培养7d时酶活达到最大值,为88.35u/Ml。
dc.description.abstractα-1,6-dextranase,which can hydrolyze dextran specifically by cutting off the α-1,6-glycosidic bond to release shorter saccharides,was widely used in many fields such as sugar industry and beer industry.The gene of α-1,6-dextranase(dex)was amplified through PCR by using Penicillium minioluteum C12114 genomic DNA as template.The amplified gene was cloned into vector pPIC9K and the recombinant plasmid pPIC9K-dex was linearzed with Sac I,then transformed into P.pastoris GS115 by electroporation.The positive transformant was induced to express the enzyme with 1.5% methanol for 7 days under the 30℃,and the activity of the enzyme could reach 88.35U/mL.
dc.description.sponsorship广东省科技计划项目
dc.language.isozh_CN
dc.subjectα-1
dc.subject6-葡聚糖酶
dc.subject朱黄青霉
dc.subject毕赤酵母
dc.subject诱导
dc.subjectα-1
dc.subject6-dextranase
dc.subjectPenicillium minioluteum
dc.subjectP.pastoris
dc.subjectinduction
dc.title朱黄青霉α-1,6-葡聚糖酶在毕赤酵母中的分泌表达
dc.title.alternativeExpression of α-1,6-dextranase from Penicillium minioluteum in P.pastoris
dc.typeArticle


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