Inhibitory effects of substrate analogues on enzyme activity and substrate specificities of mushroom tyrosinase
- 生命科学－已发表论文 
Tyrosinase can catalyze the oxidation of o-diphenols to o-quinones. In this paper, some o-diphenols were used as mushroom tyrosinase substrates to study the catalyzed specificity of the enzyme. The enzyme kinetic analysis of substrate specificities and the substrate analogues towards mushroom tyrosinase has been investigated. Taking L-3,4-dihydroxyphenylalanine (I), 3,4-dihydroxyhydrocinnamic acid (II), 3,4-dihydroxycinnamic acid (III) and 1,2,4-benzenetriol (IV) as substrates, the results of specificity studies showed that the oxidation reaction of tested o-diphenols by mushroom tyrosinase followed Michaelis-Menten kinetics. The Michaelis-Menten constants for these four substrates were determined to be 0.615, 1.238, 0.331 and 1.886mM, respectively. The values of V-m/K-m., which denotes the affinity of the enzyme to the substrate, were, determined and compared, and the results showed that the affinity of the enzyme to these substrates followed the order: compound IV > III > I > II. Furthermore, mushroom tyrosinase cannot catalyze the oxidation of 3,4-dihydroxybenzonitrile (a), 3,4-dihydroxybenzaldehyde (b), 3,4-dihydroxybenzoic acid (c) and 2,3dihydroxybenzoic acid (d). On the contrary, compounds a, b and c can inhibit the activity of tyrosinase for the oxidation of DOPA, while compound d had no effects on enzyme activity. The results show that compounds a and b are reversible non-competitive inhibitors. (c) 2006 Published by Elsevier Ltd.
CitationFood Chemistry，Volume 103, Issue 4, 2007, Pages 1075-1079