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基于液滴微流控接口的高效液相色谱-毛细管电泳二维分离平台初探
Development of a droplet-interfaced high performance liquid chromatography-capillary electrophoresis two dimensional separation platform

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基于液滴微流控接口的高效液相色谱-毛细管电泳二维分离....pdf (162.6Kb)
Date
2011
Author
叶淋泉
吴清实
戴思敏
肖志良
张博
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  • 化学化工-已发表论文 [14237]
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Abstract
蛋白质组体系的高度复杂性需要更高分辨率的多维分离技术。近年兴起的液滴技术在微纳尺度样品操控方面具有微体积、低扩散、无返混等独特优势,有望为多维分离平台的接口提供解决方案。通过采用不同结构的液滴微流控芯片可以实现“液滴生成“与“油相排除“功能,进行样品由连续流-非连续流-连续流的高效转移,将不同的分离模式进行二维耦联。本研究利用液滴作为接口技术耦联高效液相色谱与毛细管电泳构建二维分离系统,以蛋白质降解的复杂多肽混合物为样品,考察了液滴接口二维分离平台的可行性和有效性,并获得3 000以上的峰容量,初步展示了该接口技术在多维分离分析领域的应用潜力。
 
Proteomics demands high resolution multidimensional separation techniques due to its extremely high complexity.Droplet microfluidics provides a series of unique advantages in manipulating micro and nanolitre samples,such as micro-volume operation,limited diffusion and none cross-contaminating,therefore has the potential to be an ideal interface strategy for multidimensional separation.Using the microchips of different structures,functions such as "droplet generation" and "oil depletion" can be realized.Based on these functions,samples can be transferred from continuous flow to segmented flow and then back to continuous flow.In this way,different separation modes can be combined.In this study,droplet technology was utilized as a novel interface strategy in combining high performance liquid chromatography(HPLC) and capillary electrophoresis(CE).Using tryptic peptides mixture as a sample,this two dimensional HPLC-CE system provided high resolution separation with a peak capacity over 3 000.This proof-of-principle study has demonstrated the usefulness of droplet interface technology in multidimensional separation.
 
Citation
色谱,2011,(9):60-64
URI
https://dspace.xmu.edu.cn/handle/2288/106512

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