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dc.contributor.author李景喜
dc.contributor.author王小如
dc.contributor.author庄峙厦
dc.contributor.author崔维刚
dc.date.accessioned2016-05-17T02:51:33Z
dc.date.available2016-05-17T02:51:33Z
dc.date.issued2010
dc.identifier.citation光谱学与光谱分析,2010,(3):214-217
dc.identifier.issn1000-0593
dc.identifier.otherGUAN201003051
dc.identifier.urihttps://dspace.xmu.edu.cn/handle/2288/106338
dc.description.abstract研究了非放射性碘标记-电感耦合等离子体质谱免疫分析体系,该体系以兔抗大肠杆菌作为模型抗原,羊抗兔Igg蛋白为抗体,建立了一种新的免疫分析方法。实验中采用溴代琥珀酰胺(nbS)为氧化剂,实现非放射性127I标记羊抗兔Igg蛋白,探索了标记的最佳条件,标记率为63.12%;标记物在SEPHAdEX g50柱上分离纯化后,研究了I-羊抗兔蛋白的稳定性,结果表明,标记物在4℃放置96 H后几乎没有碘脱落,并保持一定的活性。实验中采用聚苯乙烯96孔板作为固相载体进行免疫反应,以电感耦合等离子体质谱为检测手段,方法的检出限为0.12 Mg.l-1,rSd(n=9)为3%;该体系也可适用于其他活性蛋白、核酸等的标记和分析。
dc.description.abstractIn the present study,the system of nonradioactive iodine-labeled-antibodies linking inductively coupled plasma mass spectrometry for immunoassay was reported.The goat-anti-Escherichia coli and goat anti rabbit were considered as simulant antigen and antibody respectively in order to establish a new method of immunoassay by inductively coupled plasma mass spectrometry which has the advantage of high sensitivity,low detection limit and preferable linearity range.During the experiment,the N-bromosuccinimide,a mild oxidant,was used to oxidize the non-radioactive iodine(127I) that labeled the protein.The method of nonradioactive iodine labeled protein was established and the best labeling condition was explored.The compound of I was purified by Sephadex G50 column chromatography,then the stability and activity were examined.The results showed that the labeling program was simple,reaction time was within two minutes,the labeling yield achieved 63.12% and none of I shed from the compound after 96 hours.The simulant antigen and antibody reacted on polystyrene microtiter plate and the I was detected by ICP-MS,the detection limit of the method was 0.12 mg·L-1,relative standard deviation(n=9) was less than 3% and the linearly dependent coefficient was 0.998 7.This system can also be used in analysis of other protein,nucleic acid and so on.
dc.description.sponsorship海洋公益性行业科研专项项目(200705011);中国海监技术支撑体系项目;2008年海洋环境保护及节能减排专项项目资助
dc.language.isozh_CN
dc.subject非放射性碘
dc.subject免疫分析
dc.subject电感耦合等离子体质谱
dc.subjectNon-radioactive iodine
dc.subjectImmunoassay
dc.subjectICP-MS
dc.title非放射性碘标记-电感耦合等离子体质谱用于免疫分析研究
dc.title.alternativeNonradioactive Iodine-Labeled Antibodies-Inductively Coupled Plasma Mass Spectrometry for Immunoassay
dc.typeArticle


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