橄榄果实提取物中没食子酸含量及自由基清除能力的研究

Abstract

采用高效液相色谱法测定橄榄果实甲醇提取物中没食子酸的含量,并用二苯基苦基苯肼自由基(dPPH.)法研究了橄榄果实提取物的自由基清除能力。没食子酸质量浓度在8.4--42 Mg/l范围内,没食子酸峰面积与其进样量呈良好的线性关系(r=0.999 3),没食子酸平均质量分数4.03%,相对标准偏差(rSd)(n=6)1.77%,平均回收率98.75%,rSd(n=6)1.21%。该方法简单灵敏,准确可靠,重复性好。橄榄果实甲醇提取物具有较高的自由基清除能力,dPPH.的半数抑制浓度(IC50)为40.14 Mg/l。

The content of gallic acid in extract of white canarytree(Canarium album(Lour.)Raeusch) fruit was determined by HPLC.The radical scavenging activity of extract was determined by 1,1-diphenyl-2-picrylhydrazyl(DPPH) assay.The linearity of gallic acid content was over the range of 8.4-42 mg/L with good linear correlation(R=0.999 3).The mean value of gallic acid content was 4.03 %,with relative standard deviation(RSD)(n=6)1.77 %.The average recovery was 98.75 % with RSD(n=6)1.21 %.The determination method is simple,sensitive,accurate,reliable and with good reproducibility.C.album fruit extract showed a very good DPPH radical scavenging activity(IC50) 40.14 mg/L.