Polymer Characteristics of Shark Liver Ferritin and Its Subunits by Electrophoresis and Mass Spectrometry

Abstract

An analytical technology from the Liver ferritin of Sphyrna zygaena (SZLF) has been modified for maintaining original interaction types both ferritin and Its Subunits for studying on the structure and function. A non-denaturing degrade polyacrylamide gel electrophoresis (ND-PAGE) was used to produce SZLF with high purity for analysis of mass spectrometry (MS). It was found that there were different polymers both SZLF and its subunits. The types and numbers of polymers were connected with the protein subunits and the separation medium. The laser come from the MALDI-TOF MS and matrix has capacities for making the subunits of ferritin into molecular ions for mass analysis, indicating that m/s of two representive peaks are 10889.35 and 22030.45, called [M + 2H](2+), with double charge and [M + H](+) with single charge. Both SDS-PAGE and NDPAGE revealed that the interaction intensity of SZLF with different polymer are higher than that of single protein itself, which resulted in the structural information of subunit from the SZLF were difficult to be determined by MALDI-TOF MS. Even the protein consists of single subunit type, the polymer interaction both SZLF and its subunits still play an important role in carrying out its physiological functions.