• 中文
    • English
  • English 
    • 中文
    • English
  • Login
View Item 
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
  •   DSpace Home
  • 生命科学学院
  • 生命科学-已发表论文
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cloning, expression and characterization of two C-type lectins from the venom gland of Bungarus multicinctus

Thumbnail
Full Text
A novel ansamycin, naphthomycin K from Streptomyces sp..htm (413bytes)
Date
2007
Author
Lin, Lu-Ping
Lin, Qun
Wang, Yi-Quan
王义权
Collections
  • 生命科学-已发表论文 [5901]
Show full item record
Abstract
C-type lectins found in many animals are non-enzymatic proteins and able to bind with mono- and oligosaccharides in a Ca2+-dependent fashion. Here, we report the cloning of two C-type lectins named BML-1 and BML-2 from the venom gland of Bungarus multicinctus, and expression of their mature peptides with 135 and 137 amino acids as inclusion bodies. Recombinant BML-1 and BML-2 proteins with 135 amino acids formed monomers, and those with 137 amino acids formed homodimers and monomers and both of them displayed certain hemagglutinating activity to rabbit erythrocytes. The results of Western blotting and immuno-affinity chromatography demonstrated that C-type lectins in B. multicinctus formed dimers in physiological conditions, and their molecular weight is lower than previous predictions. This is the first report of the cloning of the BML-2 gene from the venom gland of B. multicinctus, as well as an investigation of its confirmation and biological functions. (C) 2007 Elsevier Ltd. All rights reserved.
Citation
Toxicon,Volume 50, Issue 3, 1 September 2007, Pages 411-419
URI
http://dx.doi.org/doi:10.1016/j.toxicon.2007.04.019
https://dspace.xmu.edu.cn/handle/2288/10519

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies
 

 

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister

copyright © 2002-2016  Duraspace  Theme by @mire  厦门大学图书馆  
About | Policies